Subspecies-specific haemagglutination patterns of fimbriated Bacillus thuringiensis spores
نویسندگان
چکیده
منابع مشابه
Surface display of recombinant proteins on Bacillus thuringiensis spores.
The insecticidal protoxin from Bacillus thuringiensis has been shown to be a major component of the spore coat. We have developed a novel surface display system using B. thuringiensis spores in which the N-terminal portion of the protoxin is replaced with a heterologous protein. The expression vector with a sporulation-specific promoter was successfully used to display green fluorescent protein...
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Three species of Bacillus were evaluated as potential surrogates for Bacillus anthracis for determining the sporicidal activity of chlorination as commonly used in drinking water treatment. Spores of Bacillus thuringiensis subsp. israelensis were found to be an appropriate surrogate for spores of B. anthracis for use in chlorine inactivation studies.
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Practically all Bacillus thuringiensis strains contain a set of self-replicating, extrachromosomal DNA molecules or plasmids, which vary in number and size in the different strains. The plasmid patterns obtained from gel electrophoresis have previously been used as a tool to characterize strains, but comparison of the plasmid patterns has been limited in the number and diversity of strains anal...
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iii ACKNOWLEDGEMENTS First and foremost, I have to thank my research advisor, Prof. Ronald Labbe for his guidance, encouragement and dedicated support in every step throughout the process. The accomplishment of this paper is due to his understanding and unfaltering faith in me over the last three years. While working with him, I not only developed as a professional, but also as an individual. I...
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We report a highly sensitive, rapid and reliable method for the detection of Bacillus Anthracis spores at 300/mL using a piezoelectric-excited millimeter-sized cantilever (PEMC) sensor. Antibody specific to Bacillus anthracis (BA, Sterne strain 7702) spores was immobilized on PEMC sensors, and exposed to spores (300 to 3x10 spores/mL). The resonant frequency decreased at a rate proportional to ...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1991
ISSN: 0378-1097
DOI: 10.1016/0378-1097(91)90636-o